Cell cultures - in 3D

Bioneer, a Danish Biotech group, is using the microfluidics capabilities of Dolomite’s 'Micro Droplet System' to enable 3D cell culture growth by encapsulating cells in gelatin hydrogels.

The project is part of the Optisort Eurostars project, which sees Bioneer and Dolomite working in partnership with optical cell sorting specialist OptoRobotix to develop optically-based technologies for advanced cell sorting.

This technique relies on the formation of precisely controlled hydrogel droplets using Dolomite’s microfluidic chips and flow control technology.

Flemming Jørgensen, Project Manager at Bioneer, explained: “Encapsulation of protein-producing cells inside gelatin micro droplets offers an affordable, easy to manipulate 3D cell culture technology for non-adherent cells. The small size of the droplets makes it easy for nutrients to diffuse into the droplets from the surrounding medium, and for cellular products to diffuse out, while providing a distinct 3D environment for cell growth. This technology has potential applications for the sorting and isolation of, for example, cells with very high protein yields, and we have already shown the Chinese hamster ovary (CHO) cell cultures continue to grow within this gel matrix with good viability.”  

“Starting with a premixed cell suspension containing 6% gelatin, the Micro Droplet System is used to create droplets approximately 200 μm in diameter. Addition of a cross linker shortly before droplet production results in the formation of soft hydrogel particles containing just a few cells – or even individual cells if desired, depending on the starting concentration – which are then resuspended in fresh cell medium."