Membrane chromatography capsules redesigned

Sartorius Stedim Biotech has redesigned its Sartobind membrane chromatography capsules to more effectively recover large molecular weight proteins such as blood factors and conjugated proteins or viruses

A range of capsule sizes with a 4 mm bed height is now available from the 1 ml nano unit up to the new 2.5 l jumbo version.

The capsules’ upstream flow channels have been optimised and an internal core forms a miniaturised downstream channel, resembling that in existing 8 mm bed height capsules. The new Q, S and salt-tolerant STIC PA ion exchanger capsules, all with a 4 mm bed height, increase dynamic binding capacity by approximately 15% and reduce void volumes by approximately 40% compared with their predecessors, while maintaining high flow rates of 10 to 30 membrane volumes per minute.

By improving breakthrough behaviour, subsequent polishing steps to remove DNA, HCP, aggregates and viruses from recombinant proteins are significantly more reliable and, as less buffer is used, Sartobind also reduces operational costs.

Furthermore, Sartorius Stedim Biotech has added new 400 and 800 ml Sartobind Q, S and Phenyl capsules with an 8 mm bed height to its portfolio. These optimised capsules increase dynamic binding capacity up to 48% when compared to adsorbers installed in filter housings.