New study shows true impact of endotoxins in 3D in vitro models
Darling Ingredients’ health brand Rousselot, a global supplier in collagen-based solutions, announced that a new study has found that utilisation of highly purified gelatin biomaterials for 3D in vitro models can significantly enhance the reliability and reproducibility in these models, creating new possibilities for replacing preclinical animal trials.
Key highlights:
- A joint study by the University of Twente, The Netherlands, and Rousselot investigated for the first time, the impact of endotoxins on 3D breast tumor-immune cancer models.
- The study measured the effect of the interaction between macrophages and cancer cells, as well as the measured efficacy of immunotherapies.
- Recent changes in the EU and US FDA legislation, combined with increased ethical considerations and efficacy demands, are driving the rapid replacement of pre-clinical animal trials with 3D biofabrication models.
“This study revealed that 3D models of novel breast cancer immunotherapies display clear therapeutic efficacy differences depending on the level of endotoxins in gelatins and methacryloyl gelatins (GelMA) used in in vitro systems. Biomaterials with low endotoxin content could lead to a more accurate representation of the safety and potency of novel therapeutics. This can improve the validity of 3D in vitro models and help to reduce animal testing,” said Dr. Kathleen Jacobs, global regulatory affairs director, Rousselot.
The joint study done by the University of Twente, The Netherlands, and Rousselot Biomedical investigated for the first time the impact of endotoxins on 3D breast tumor-immune cancer models. The research compared 3D models using standard gelatins and GelMAs or Rousselot’s X-PURE gelatin and X-PURE GelMA – purified biomaterials with low endotoxin levels (<10 EU/g).
The study demonstrated that endotoxin levels had a marked effect on the validity of the 3D-printed model in three aspects:
- Inflammatory reaction: The presence of high levels of endotoxins caused a strong inflammatory response in immune cells (macrophages) in contaminated models.
- Receptivity of immune cells to cancer cells: Crosstalk between macrophages and cancer cells was significantly reduced in the presence of high endotoxin levels.
- Reliability of therapeutic outcomes: The high endotoxin environment artificially increased the measured efficacy of the therapy designed to inhibit the expression of anti-inflammatory markers.
Recent changes in European Union and US Food and Drug Administration (FDA) legislation, combined with increased ethical considerations and efficacy demands, are driving the rapid replacement of pre-clinical animal trials with 3D biofabrication models.
Dr. Bjorn Vergauwen, scientific director, product and process development, Rousselot concluded: “The use of purified biomaterials such as Rousselot’s X-Pure or X-Pure GelMA in in vitro 3D models is critical in producing a biologically relevant environment, while also helping to reduce the costs, time and ethical concerns associated with drug development.”