Protein quality control in high-throughput screening

Wyatt Technology has published a whitepaper outlining how to evaluate the solution quality of biotherapeutic candidates using the DynaPro plate reader II prior to screening via surface plasmon resonance (SPR) or bio layer interferometry (BLI).

Analyte quality is critical to obtaining accurate and meaningful measurements in order to select the most promising therapeutic candidates, so ensuring the quality of samples is a vital step in the screening process. The DynaPro high-throughput dynamic light scattering (HT-DLS) system provides an effective method of assessing sample materials using a non-invasive, non-pertubative optical technique.

SPR and BLI are popular techniques for high-throughput screening and discovery of biotherapeutic candidates. Impurities such as aggregates and foreign particles often impact measurements through noise, spurious signals and inaccurate active concentrations. In addition, owing to their size and the small apertures of the on-chip channels, particulates may cause blockage to occur in sensitive SPR microfluidics.

Dr. Daniel Some, principal scientist at Wyatt Technology, commented: "Guaranteeing that samples are appropriate for SPR, BLI or ITC analyses is critical to proper binding screens. The rapid and automated quality assessment provided by the DynaPro Plate Reader II HT-DLS system ensures a valuable layer of quality control that would simply not be an option with manual, cuvette-based testing. When single binding experiments are needed, the DynaPro NanoStar microcuvette DLS system evaluates solution quality with as little as one microliter of sample.”

Requiring no fluidic handling, the DynaPro Plate Reader II HT-DLS system can quickly and effectively analyse protein quality in large sample populations with no concerns for carryover between samples. By automatically classifying samples as high, medium and low quality, the DynaPro, in conjunction with DYNAMICS data analysis, collection and reporting software, provides intuitive visual analysis to quickly evaluate the suitability of each solution for reliable binding screens.

The DynaPro performs measurements in the same microwell plates as SPR and BLI high-throughput discovery screening platforms, saving researchers considerable time and effort in assessing the quality of large batches of biotherapeutic samples and ensuring accurate results.