Sartorius Stedim Biotech releases modular chromatography solution

Supplier for the biopharmaceutical industry, Sartorius Stedim Biotech (SSB) has announced an expansion of its range of single-use membrane chromatography solutions with the release of Sartobind Cassettes.

The pod-like modular systems have been developed for commercial applications in both capture and polishing. Capture of large proteins such as viruses and virus-like particles (VLPs), protein conjugates and blood factors is important in bioprocessing.

“For the first time, the high binding capacity of membrane adsorbers can be used at almost any scale in commercial vaccine manufacture,” said Dr Stefan Fischer-Frühholz, membrane chromatography expert at Sartorius Stedim Biotech. “Now, a 200 L Q anion exchange column for adenovirus capture can be easily replaced by a 20 L Q adsorber cassette system.”

The cassettes offer the same flow path, bed heights (4 and 8 mm) and void volume ratios as the Sartobind capsules and are compatible with Q, S, STIC PA and phenyl ligands. Multiple cassettes, each with 0.8 or 1.6 L membrane volume, can be set up in three different stainless steel holders resulting in maximum volumes of 20, 50 or 100 L, respectively. The company reports that pressure-flow performance and the shape of breakthrough curves are identical to the smaller capsule sizes, independent of the number of cassettes used.