Depth Filters Deliver Faster Clarification of Pretreated Feed Streams

Merck Millipore, the Life Science division of Merck, has introduced the Clarisolve depth filter for single-stage clarification of pretreated feed streams. The Clarisolve depth filter has a gradient density structure specifically designed to the particle size distributions of pretreated feed streams. 

Delivering improved volumetric capacity and reduced turbidity over any currently available depth filtration technologies, Clarisolve depth filters process pretreated feeds in a significantly reduced footprint without the need for the secondary stage of clarification. The use of Clarisolve depth filters eliminates the need for centrifugation, which enables implementation of a fully single-use process train, and also considerably reduces the pre-use flushing requirements. This technology was developed to address the shortcomings of traditional downstream clarification approaches when processing high cell density and high-product titer cell cultures.  

“Until now, higher titers coming from upstream bioreactors have posed a challenge to conventional approaches to clarification,” described Vin Donovan, Vice President of Biopharm Process Solutions, Merck Millipore. “With Clarisolve depth filters, our customers can now take full advantage of high density pretreated feed streams and improve overall process economics.”

Merck Millipore has also developed a cationic pDADMAC (poly diallyldimenthyl ammonium chloride) flocculation reagent targeted for biopharmaceutical applications, which can be used as a pretreatment in combination with the Clarisolve depth filters. pDADMAC treatment, in combination with Clarisolve depth filters, provides an effective solution for harvesting high cell density cultures and can be easily incorporated into current clarification platforms.

Clarisolve filters are available from laboratory to pilot and process scale in the popular disposable Pod format.

Merck Millipore, +1 781 533 5336, pegeen.dossantos@emdmillipore.com, www.merckmillipore.com.