Cambio
Cambio is able to supply its customers with a selection of reagents by Microzone. MegaMix, microLYSIS and MicroCLEAN are each specifically designed as convenient and efficient solutions for PCR, DNA release and DNA clean-up respectively. By incorporating these reagents into their procedures, life scientists can save time that would otherwise be spent creating their own mixes as well as reducing risk of contamination. Thanks to their standardised formulation, the Microzone reagents also dramatically increase the reproducibility of DNA amplification, giving researchers the upmost confidence in their methods and therefore their results.
MegaMix is a PCR master mix containing all the fixed components needed for PCR amplification, including an enzyme cocktail consisting of two Taq polymerases, dNTPs, MgCl2, reaction buffers and enzyme stabilisers. All these components are optimised to maximise yield with minimal non-specific background and the product is exceptionally easy to use — the MegaMix just has to be thawed, the recommended volume drawn and DNA and primers added. MegaMix can survive multiple freeze/thaw cycles without deterioration of either product or function. MegaMix is also available with dye to allow easy visualisation and double concentrated for added convenience.
In addition, Cambio also supplies Microzone’s MicroCLEAN and microLYSIS products. MicroCLEAN is a rapid, one tube, one and a half spin DNA reagent, which cleans up any double stranded DNA from reaction buffers, enzymes, primers or dNTPs. The versatile reagent has no limit on the size or volume of the target DNA and requires no additional manipulation such as phenol extractions or spin columns.
Finally, microLYSIS and microLYSIS-Plus are reagents designed for use as a pre-step to PCR. When combined with rapid heating and cooling of thermocyclers, the mycroLYSIS formulation effectively lyses the cells and enables users to reach the PCR stage in less than 15 minutes. For tougher cell samples, such as those found in plant and some animal tissues, microLYSIS-Plus can still offer the same impressive results.
Cambio Ltd, +44 1954 210200, support@cambio.co.uk, www.cambio.co.uk.